Selenium Recovery and Conversion by a Filamentous Fungus, Aspergillus oryzae Strain RIB40
Abstract
A filamentous fungus (koji mold), Aspergillus oryzae strain RIB40 could be applied for selenium recovery and conversion. The granular cell-aggregates (mycelial cells) were able to reduce and convert selenite into nano-sized elemental selenium depositions when the granules were incubated under aerobic condition shaking at 120 rpm with a 100 ml baffled Erlenmeyer flask contained 20 ml of PD (potato dextrose) medium. In the aerobic condition, selenite (1 mM) was removed completely (over 99 %) from the culture within 24 hours by the mycelial cell-granules of strain RIB40. The cells turned red-orange due to the formation of elemental selenium nanospheres. Results of total selenium quantity determination in the red-orange coloured cellular granules by the atomic absorption method showed that most of selenite which disappeared from the culture were recovered to the granular cell-aggregates. These results demonstrate that a filamentous fungus, Aspergillus oryzae strain RIB40 can be used as an effective bio-material for recovery and reduction, and conversion of selenium-oxyanion in bioremediation, elemental resource recycling, biomineralization and biofabrication.
Keywords— Aspergillus oryzae, Selenite reduction, Selenium recovery.
References
Zhang, J., Zhang, S.Y., Xu, J.J. and Chen, H.Y. 2004. A new method for the synthesis of selenium nanoparticles and the application to construction of H2O2 biosensor. Chinese Chem. Lett. 15: 1345-1348.
Haygarth, P.M. 1994. Global importance and global cycling of selenium, In Frankenberger Jr., W.T. and Benson, S. (Eds). Selenium in the environment, pp. 1-27. New York: Marcel Dekker Inc.
Conde, J.E. and Sanz Ajeajos, M. 1997. Selenium concentration in natural and environmental waters. Chem. Rev. 97: 1979-2003.
Doran, J.W. 1982. Microorganisms and the biological cycling of selenium. Adv. Microb. Ecol. 6: 1-32.
Bodnar, M., Konieczka, P. and Namiesnik, J. 2012. The properties, functions and use of selenium compounds in living organisms. J. Enrion Sci. Health C Environ. Carcinog. Ecotoxicol. Rev. 30: 225-252.
Araie, H. and Shiraiwa, Y. 2009. Selenium utilization strategy by microalgae. Molecules 14: 4880-4891.
Sors, T.G., Ellis, D.R. and Salt, D.E. 2005. Selenium uptake, translocation, assimilation and metabolic fate in plants. Photosynth. Res. 86: 373-389.
Rayman, M.P. 2012. Selenium and human health. Lancet 379: 1256-1268.
Kieliszek, M. and Błażejak, S.B. 2013. Selenium: Significance, and outlook for supplementation. Nutrition 29: 713-718.
Schrauzer, G.N. 2006. Selenium yeast: composition, quality, analysis and safety. Pure Appl. Chem. 78: 105–109.
Painter, E.P. 1941. The chemistry and toxicity of selenium compounds, with special reference to the selenium problem. Chem. Rev. 28: 179-213.
Lemly, A.D. 2004. Aquatic selenium pollution is a global environmental safety issue. Ecotoxicol. & Environ. Safety 59: 44-56.
Machida, M., Yamada, O. and Gomi, K. 2008. Genomics of Aspergillus oryzae: Learning from the history of koji mold and exploration of its future. DNA Res. 15: 173–183.
Machida, M., Asai, K., Sano, M., Tanaka, T., Kumagai, T., Terai, G., Kusumoto, K., Arima, T., Akita, O., Kashiwagi, Y., Abe, K., Gomi, K., Horiuchi, H., Kitamoto, K., Kobayashi, T., Takeuchi, M., Denning, D.W., Galagan, J.E., Nierman, W.C., Yu, J., Archer, D.B., Bennett, J.W., Bhatnagar, D., Cleveland, T.E., Fedorova, N.D., Gotoh, O., Horikawa, H., Hosoyama, A., Ichinomiya, M., Igarashi, R., Iwashita, K., Juvvadi, P.R., Kato, M., Kato, Y., Kin, T., Kokubun, A., Maeda, H., Maeyama, N., Maruyama, J., Nagasaki, H., Nakajima, T., Oda, K., Okada, K., Paulsen, I., Sakamoto, K., Sawano, T., Takahashi, M., Takase, K., Terabayashi, Y., Wortman, J.R., Yamada, O., Yamagata, Y., Anazawa, H., Hata, Y., Koide, Y., Komori, T., Koyama, Y., Minetoki, T., Suharnan, S., Tanaka, A., Isono, K., Kuhara, S., Ogasawara, N. and Kikuchi, H. 2005. Genome sequencing and analysis of Aspergillus oryzae. Nature 438: 1157-1161.
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