Chemical Composition and Protein Quality of Fish Sauces (Kecap Ikan and Nampla)
Abstract
Chemical analyses were carried out on two commercial fish fermented products; kecap ikan which made from Indonesia and nampla from Thailand. Chemical composition, colour and amino acid composition were determined and the result shows the significant difference (p<0.05) in the parameters between two commercial fish sauces. Protein content in kecap ikan and nampla were 1.60 and 10.85 % respectively. Degree of protein hydrolysis was determined by precipitating the soluble nitrogen in 10% trichloroacetic acid (TCA) with 64.62% for kecap ikan which higher (p<0.05) than 56.10% degree hydrolysis in nampla. To assess the protein quality of these products, amino acid composition was determined. Total amino acid constitute for 707.8 mg/100mL protein in kecap ikan and nampla containing 8480.3 mg/100mL protein. Chemical score, amino acid score and essential amino acid index were 0.00, 0.00 and 0.56 for kecap ikan and 4.22, 5.88 and 7.32 for nampla. Protein digestibility is high in kecap ikan and nampla with 66.51 and 66.26% respectively. Protein digestibility-corrected amino acid score value was only 10.70 % in kecap ikan while 100% in nampla. The quality of protein in nampla indicates that it can provide complete essential amino acid to human. However, the quantity of protein and amino acid was not significant as source of essential amino acid in human diet because of limited daily intake.
Keywords— Fermented fish product, fish sauce, kecap ikan, nampla, protein quality
References
Acton, J.C.and C.L. Rudd, (1987) Protein Quality Method for Seafood. In: Seafood Quality Determination, Kramer, D.E. and J. Liston (Eds.). Elsevier, Amsterdam, pp: 453-473.
A.O.A.C (1990) Official Methods of analysis of the association of official analytical chemistry (15th ed.). Arlington, VA: Association of Official Analytical Chemists.
A.O.A.C (2000) Official Methods of analysis of the association of official analytical chemistry (17th ed.) AOAC International: Gaithersburg, MD.
Amano, K. (1962). Influence of fermentation of the nutritive value on fish with special reference to fermented fish products of Southeast Asia. In E. Heen & R. Kreuzer (Eds.), Fish in nutrition (pp. 180–200). London: Fishing News
AOAC International. (1995) Official methods of analysis of AOAC International. 2 vols. 16th edition. Arlington, VA, USA, Association of Analytical Communities.
Badan Standarisasi Nasional. (1996) Standar Nasional Indonesia (SNI). SNI 01-4271-1996.Produk Kecap Ikan. Jakarta : Dewan Standarisasi Indonesia.
Baens-Arcega, L (1977) Patis, a traditional fermented fish sauce and condiment of the Philippines. Symposium of Indigenous Fermented Foods, Bangkok, Thailand.
Barbut, S., Tanaka N., Maaurer A.J.,(1986) Effect of varying levels of chloride salt on Clostridium botulinum toxin production in Turkey Frankfurters, J Food Sci, 51(5) 1129-1131.
Brillantes, S. (1999) Histamine in fish sauce-health and safety considerations, Infofish International, 4, 51–56.
Clatterbuck, K.L., Kehrberg, N.L.,and Marable, N.L. (1980) Journal of food science 45: 935
Damodaran, S. (1996). Amino acids, peptides and proteins. In: Fennema O.R. (Ed.) Food Chemistry (pp. 321-429) 3rd edition, New York: Marcel Dekker.
Davidson, P.M.,(1997) Chemical preservatives and natural antimicrobial compounds, In: Food Microbiology-Fundamentals and Frontiers, edited by Doyle MP, Beuchat LR & Montville TJ, (ASM Press, Washington DC).
Eicher, N., (1982) The chemical and nutritional characteristics of great northen bean protein as affected by pH and heat, MS thesis, University of Nebraska –Lincoln.
El, S. N., and Kavas, S.N. 1996. Determination of protein quality of rainbow trout (Salmo irideus) by in vitro protein digestibility – corrected amino acid score (PDCAAS). Food Chemistry, 55(3): 221-223).
Caplice, E., (1999) Food fermentations: role of microorganisms in food production and preservation, International Journal of Food Microbiology 50, 131–149.
FAO/WHO (1991) Protein Quality Evaluation; Food and Agricultural Organization of the United Nations: Rome, Italy,p 66
FAO/WHO/UNU WHO (1985). Energy and Protein requirement. Report of a Joint FAO/WHO/UNU Expert Consultation, Tech. Rept.Series No. 724, WHO, Geneva, Switzerland.
FAO/WHO/UNU WHO (2007). Protein and amino acid requirements in human nutrition. Report of a Joint FAO/WHO/UNU Expert Consultation, Tech. Rept.Series No. 935, WHO, Geneva, Switzerland.
Frazier, W.C., Westhoff, D.C., (1988) Food Microbiology. McGraw Hill, New York.
Gildberg, A. (2001). Utilization of male Arctic capelin and Atlantic cod intestines for fish sauce production–evaluation of fermentation conditions. Bioresource Technology, 76, 119–123.
Haslaniza H., Maskat, M.Y., Wan Aida, W.M., Mamot, S. (2010) The of enzyme concentration, temperature and incubation time on nitrogen content and degree of hydrolysis of protein precipitate from cockle( Anadara granosa ) meat wash water, Int. Food Research Journal 17:147-152
Hoyle, N.T.and Merritt, J.H. (1994) Quality of fish protein hydrolysates from herring ( Clupea harengus). Journal of food Science 59(1): 76-79.
Hsu, H.H., Vavak, D. L., Satterlee L.D., Miller., G.A. (1977) A multienzyme technique for estimating protein digestibility, J.F.S. 42: 1269-1273.
Huda, N., (2012) Indonesian Fermented Fish Products In: Handbook of Animal-Based Fermented Food and Beverage Technology, Second Edition,Y . H . Hui and E . Özgül. Evranuz CRC Press 2012 Pages 717–738.
Ismail, M.S., (1977) Accelerated fermentation of fish soy paste and fish soy-sauce by using Aspergillus oryzae NRRL 1989. Symposium on Indigenous Fermented Foods, Bangkok, Thailand.
Kawashima Kand Yamanaka H. (1996) Free amino acids responsible for the browning of cooked scallop adductor muscle. Fisheries Science 62(2): 293-296.
Lopetcharat, K.and Park, J. W. (2002). Characteristics of fish sauce made from Pacific whiting and surimi by-products during fermentation storage. Journal of Food Science, 67, 511–516.
Lopetcharat, K., Choi, Y.J., Park, J.W., Daeschel, M.A. (2001). Fish sauces products and manufacturing –a review. Food International, 17(1), 65-88.
McDonough, F. E.; Steinke, F. H.; Sarwar, G.;Eqqum, B.O.;Bressani, R.; Huth, P.J.;Barbeau, W.E.;Mitchell,G.V.;Philips, G.J.(1990) In vitro rat assay for true protein digestibility: Collaborative study. J Assoc. Off. Anal. Chem.73:801-805
Millward D. J., Layman D. K., Tome D., Schaafsma G. (2008) Protein quality assessment: impact of expanding understanding of protein and amino acid needs for optimal health. Am. J. Clin. Nutr. 87, S1576–S1581.
Mizutani, T., Kimikuza, A., Ruddle, K., Ishige, N. (1992). Chemical components of fermented fish products Journal of Food Composite Analysis, 5, pp. 152–159
Monawar, L.Y., (1983) Food value of Sudanese indigenous cereal grains. Ph.D. Thesis, University of Khartoum, Sudan.
Orejana, F. M., Liston, J. (1982). Agents of proteolysisand its inhibition in Patis (Fish sauces) fermentation, J of Food Sci, 47, 198-203.
Park, J.N., K. Ishida, T. Watanabe, (2002) Taste effects of oligopeptides in a Vietnamese fish sauces. Fisheries Science 68:921-928.
Park, J.N., Y. Fukumoto, E.Fijita, (2001) Chemical composition of fish sauces produced in Southest and East Asian countries. Journal of Food Composition Analysis 14:113-125.
Phithakpol,B,Varanyanond,W.,Reungmaneepaitoon, S., Wood, H (1995) The traditional fermented foods of Thailand. ASEAN Food Handling Bureau, p 3.
Poernomo, A. , Suryaningrum, T.D. Ariyani, F., Putro, S. (1984) Studies on the nutritive value and microbiology of traditional fishery products. Laporan Penelitian Teknologi Perikanan 30: 9-19 (in Indonesian)
Putro, S., (1993) Fish Fermentation Technology in Indonesia. In: Fish Fermentation Technology. Lee, CH, Steinkraus, K H and Alan Reilly, P J (Eds). United Nations University Press, Tokyo, Japan p 107-128.
Saisithi, P. (1994). Traditional fermented fish: fish sauce production. In A. M. Martin (Ed.), Fisheries processing biotechnology application (pp. 111–131). London: Chapman and Hall.
Sarwar, G.; Christiansen, D.A.; Finlayson, A.T.; Friedman, M. ; Hackler, L.R.; Mc Keinzie, S.L.; Pellet, P.L.; Tkachuk, R.(1983) Intra- and inter- laboratory variation in amino acid analysis. J. Food Sci. 48, 526-531.
Satterlee, L.D., Kendrick, J.G., Miller, G.A., (1977) Rapid in vitro assays for estimating protein Quality, Food Technol., 31(6) 78-81.
Satterlee, L.D. Marshall, H.F. and J.M. Tennyson. 1979. Measuring protein quality. J.A.O.C.S. 56:103-109.
Sikorski, Z.E., Gildberg,A., Ruiter, A. (1995). Fish products, pp. 315-346. In A. Ruiter, (ed.). Fish and Fishery Products: Composition, Nutritive Properties and Stability. CAB International. Wallingford, UK.
Sikorski, Z.E., Lolakowska, A., Pan, B.S., (1990) The Nutritive Composition of the Major Groups of Marine Food Organisms. In: Resources Nutritional Composition and Preservation, Sikorski, Z.E. (Ed.). CRC Press-Inc., Boca Raton, Florida, pp: 30-52.
Suzuki, T., (1981) Fish and Krill Protein Processing Technology, pp. 10-13. Applied Science Publishers, London.
Thai Industrial Standard. 1983. Local Fish Sauces Standard, Department of Industry: Bangkok, Thailand, 1983.
Tran, T. D., (2002). Country Report-Vietnam. In: Third Regional Workshop on the application of HACCP in the fish processing industry in Southeast Asia. Yeap, S E and Hariono, I (Eds) p 40.
Tsai, Y.-H., Lin, C.-Y., Chien, L.-T., Lee, T.-M., Wei, C.-I., Hwang, D.-F. (2006) Histamine contents of fermented fish products in Taiwan and isolation of histamine-forming bacteria, Food Chemistry, 98, 64–70.
Tyn, M. T., (1993) Trends of fermented fish technology in Burma. In: Fish Fermentation Technology. Lee, C H, Steinkraus, K H and Alan Reilly, P J (Eds). United Nations University Press, Tokyo, Japan p 129-153.
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